| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Gramella forsetii | TGGGCAGAATCACAGCAGG | TGCATCACCCTGGTTCTGG | 60.00 | 59.62 | 280 | 116 |
100.00%
|
100.00%
|
| Primer 12 | Gramella forsetii | ACCCTGGAACTCAGTGCAC | AGATCATGCGGTCGTTTTCC | 59.55 | 58.63 | 248 | 116 |
100.00%
|
100.00%
|
| Primer 13 | Gramella forsetii | AGCCGATGCGCTTAAAGC | CGCGATCGGGAAATTCTGC | 58.90 | 59.72 | 218 | 115 |
100.00%
|
100.00%
|
| Primer 14 | Gramella forsetii | ACCCTGGAACTCAGTGCAC | CGTCCGCAGATAGATCATGC | 59.55 | 58.66 | 259 | 115 |
100.00%
|
100.00%
|
| Primer 15 | Gramella forsetii | TGCCCTGGCGATCATTCTG | AAGCTGCTCGATCGTCTGG | 60.15 | 59.86 | 185 | 112 |
100.00%
|
100.00%
|
| Primer 16 | Gramella forsetii | TGGCAAAATGGGCTGCTG | TGCATCTGCCCAGTCAAGG | 58.94 | 60.00 | 179 | 112 |
100.00%
|
100.00%
|
| Primer 17 | Gramella forsetii | ACAAGGAGACTCTGGGCTG | TGGTCGCTTGTTCTCCTGG | 58.63 | 59.63 | 282 | 112 |
100.00%
|
100.00%
|
| Primer 18 | Gramella forsetii | ATCGCTCGCCAGAAGACTG | AGCACCTTCATTCGGCTGG | 59.86 | 60.38 | 151 | 112 |
100.00%
|
100.00%
|
| Primer 19 | Gramella forsetii | TGATGTGGTTGGAGGCCTG | AAGTGCGGTACCAAGGTGG | 59.62 | 59.93 | 223 | 112 |
100.00%
|
100.00%
|
| Primer 20 | Gramella forsetii | TCCGGTTACAAGGCGATGG | TGCCTGTCGGTTAGTCTGG | 59.78 | 59.03 | 155 | 109 |
100.00%
|
100.00%
|